Solution based post-processing methods for mycological biopolymer material and mycological product made thereby

ABSTRACT

A mycological biopolymer material is subjected to treatment in one or more solutions that work to enhance and/or retain the inherent material properties of the material. In one embodiment, the solution is an organic solution; in another embodiment, the solution is an organic solvent with a salt; in another embodiment, the solution is an organic solvent phenol and/or polyphenol; and in another embodiment, a series of such solutions is used.

INCORPORATION BY REFERENCE TO ANY PRIORITY APPLICATIONS

This application is a continuation application of U.S. patent application Ser. No. 15/940514, filed Mar. 29, 2018, which claims the benefit of Provisional Patent Application 62/479,521, filed Mar. 31, 2017, the entireties of which are hereby expressly incorporated by reference herein.

BACKGROUND OF THE INVENTION Field of the Invention

This invention relates to a processed mycological biopolymer material and a method of making the same. More particularly, this invention relates to a processed mycological biopolymer material made entirely of fungal mycelium. Still more particularly, this invention relates to a method of enhancing the material properties of a mycological biopolymer product

Description of the Related Art

As described in US Patent Application Publication 2015/0033620, published Feb. 5, 2015, a mycological biopolymer for use in making functional products may be made entirely of mycelium without producing a stipe, cap, or spores. As described, the produced mycological biopolymer may be used in structural composite cores, athletic workout mats, apparel such as handbags, shoe soles and the like.

SUMMARY OF THE INVENTION

It is an object of this invention to provide a mycological biopolymer of increased elasticity, strength and density as compared to previously known mycological biopolymers.

It is another object of the invention to provide a processed mycological biopolymer material that is a tough pliable material that can be used to replace textiles, leather, and leather-like materials, such as, polyurethane, silicone, and poly vinyl acetate coated scrims.

It is another object of the invention to provide a processed mycological biopolymer material that provides a high density foam-like material for use in upholstery, apparel, military gear, athletic gear, and footwear.

Briefly, the invention provides a processed mycological biopolymer material characterized in being entirely composed of fungal mycelium free of any stipe, cap, or spores and in having a Young's Modulus of elasticity of 2000-8000 psi and a density of from 15 pcf to 50 pcf.

In addition, the invention provides a method of making an improved processed mycological biopolymer material that resides in treating a known mycological biopolymer material (“tissue”) with one or more solutions that function to enhance the inherent material properties of the material. In this instance, the treatment fixes the tissue, making the tissue more durable to repeated stress, resistant to microbial decay, and resistant to shear stress (tearing). This treatment retains the properties of the extracted mycelium (wet) over tissue that has been actively dried which has been shown to embrittle the material.

In one embodiment, the method comprises the steps of obtaining a panel of a mycological biopolymer material (“tissue”) as a precursor material and treating the panel with an organic solvent solution for a period of time, e.g., of from 5 seconds up to 6 months, sufficient to permit permeability into the tissue which is inherently hydrophobic. This latter step slowly desiccates the precursor tissue replacing water with the solvent and any inorganics in the solvent solution.

This is rinsing away of soluble extra cellular matrix components (carbohydrates, proteins), and could denature proteins in the tissue. Further, this method could deacetylate the structural chitin matrix, which would mediate crosslinking between polymers. As is known, chitin is a primary component of cell walls in fungi and is composed of a long-chain polymer of N-acetylglucosamine, a derivative of glucose.

A byproduct of this method is the bleaching of the mycelium and elimination of odor.

After treating the precursor tissue with the organic solvent solution, for example a bath of 100% alcohol, the tissue is removed from the bath and immediately pressed to a minor fractional thickness of the original thickness and thereafter dried to a moisture content between 15-30% by dry mass.

The precursor tissue of mycological biopolymer material (as well as the processed tissue) is characterized in being entirely composed of fungal mycelium free of any stipe, cap, or spores. For example, the material may be made as described in US Patent Application Publication 2015/0033620 or as described in US Provisional Patent Applications 62/707,704, filed Nov. 14, 2017, the disclosures of which are incorporated herein. For example, the precursor tissue may be grown as described and then removed as a one-piece panel to be post-processed or the precursor tissue may be left in place on the substrate from which the tissue is grown and post-processed.

A precursor tissue of mycological biopolymer material made as described in US Patent Application Publication 2015/0033620 that has dimensions of 18 inches by 11 inches and a thickness of 2.5 inches typically has a density of 0.8 pcf to 3.0 pcf and a Young's Modulus of elasticity of 95 psi. After treatment, the thickness of this highly lofted tissue is reduced, for example, by 20 times to 0.125 inch and the density increased proportionately. In addition, the porosity of the tissue is an average of 3.4 micrometers with a range of from 0.9 to 25 micrometers.

The post-processed mycological biopolymer material is distinguished from one that is not processed in being denser, and in having a native moisture content greater that 15%, while the native precursor tissue is less than 12%.

In a second embodiment, the t precursor issue of mycological biopolymer material is treated with a solution of an organic solvent combined with a salt, such as calcium chloride, for up to six months. The use of salt imparts antimicrobial properties and can ionically bond to functional groups.

In a third embodiment, the precursor tissue of mycological biopolymer material is treated with a solution of an organic solvent combined with a phenol and/or polyphenol substance for up to six months' time.

In a fourth embodiment, the precursor tissue of mycological biopolymer material is treated with a solution of an organic solvent combined with a phenol and/or polyphenol substance as well as with a solution of an organic solvent combined with a salt for up to six months' time.

Treating the mycological biopolymer with one or a combination of organic solvent solutions, calcium chloride solutions, and phenol/polyphenol solutions greatly enhances the material's inherent strength characteristics. These treatments increase the density, ultimate tensile strength, and strength to weight ratio of the precursor mycological biopolymer material. These treatments also impact the material's elastic modulus, resulting in increased elasticity, reduction in stiffness as compared to the weight and tensile strength of the mycelium. With the application of such post-processing treatments, the ability to produce a processed tissue of mycological biopolymer material with a wider range of densities (.1.§-50 pcf) is readily achievable. The result of these enhanced material properties (increased density, strength, and elasticity) is the ability for the processed mycological biopolymer material to be competitive in industries and applications where high density foams, leather, and durable plastic textiles are currently used.

There is a variety of literature and research on the treatment of mycelial tissue with calcium chloride solutions, alcohols, and tannins. The treatment of mycelial tissue post-growth with calcium chloride solutions is common and has been successfully used for a variety of purposes such as increasing the material strength of the commercially valuable Agaricus bisporus. See, Zivanovic, S., and R. Buescher. “Changes in Mushroom Texture and Cell Wall Composition Affected by Thermal Processing.” Journal of Food Science 69 (2004): 44-49; as well as in the packaging and preserving of edible mushrooms. See U.S. Pat. Nos. 6,500,476 and 5,919,507.

Unlike previous applications of calcium chloride solutions to mycelium tissue post growth, the treatment processes described herein are intended for use on mycological biopolymer materials and not for the purpose of producing, altering, or preserving a food item or medicinal substance.

Alcohols, polyphenols; and calcium chloride are used on mycelium for extracting, synthesizing, and the like, a variety of substances. See U.S. Pat. Nos. 6,726,911; 3,268,606 and 6,482,942.

The use of alcohols, polyphenols, and calcium in the post-processing methods applied to mycological biopolymers in accordance with the invention differ from the prior art as there is no extraction or synthesizing of molecular substances intended for medicinal, pharmaceutical, cosmetic, or other such applications

BRIEF DESCRIPTION OF THE DRAWINGS

These and other objects and advantages of the invention will become more apparent from the following detailed description taken in conjunction with the accompanying drawings wherein:

FIG. 1 schematically illustrates a tissue of mycological biopolymer material submerged in a salt/solvent solution in accordance with the invention;

FIG. 2 schematically illustrates a tissue of mycological biopolymer material submerged in a tannic acid/water solution in accordance with the invention;

FIG. 3 illustrates a processed tissue being pressed in accordance with the invention;

FIG. 4 illustrates a processed mycological biopolymer material made in accordance with the invention being twisted; and

FIG. 5 illustrates a flow diagram of a process in accordance with the invention.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENT

In the embodiment employing an organic solvent solution, the following steps are performed:

-   -   A panel of wet live tissue or dried tissue, i.e., a precursor         tissue, with or without the growing substrate can be used.     -   The tissue can be treated with lipids and/or         moisturizing/hydrating agent once or repeatedly, or left         untreated at any point throughout this process.     -   The tissue can be sectioned or left intact to allow for a         variety of manufacturing sizes.     -   The tissue can be treated (via submersion, vacuum infusion,         and/or injection) once or repeatedly. For each treatment, for         every 1 g of panel, 5 to 50 ml of organic solvent solution is         applied for 5 seconds to 6 months. In this respect, the tissue         could also be treated while still growing from a substrate and,         as such, would be tethered to the substrate.

Treatment of the tissue with an organic solvent solution is for a period of time sufficient to permit permeability into the tissue while desiccating the tissue replacing native water with the solvent solution.

Increasing time permits more homogenous infiltration of the solution which, in turn, bolsters the chemical treatments.

-   -   The tissue is thereafter compressed to a minor fraction (i.e.,         less than ½) of the original thickness, for example to about         1/20 of the original thickness, using a manual press, hydraulic         press, or rollers. If treated to this point while still tethered         to the substrate, the tissue is removed from the substrate for         pressing. Pressing can be a hot (140° F.) or cold process. This         is a means of mechanically expelling any of the residual fluid         and setting the thickness, since the mycelium can plump during         treatment. It is important to set the thickness immediately         following the treatment in the organic solution to reduce         rebound and shrinkage (e.g., fixation)     -   After compression, the tissue can be dried using a convection         oven, can be freeze dried, air dried, or conductively dried     -   The tissue can be treated with a plasticizer which could include         glycerin, sorbitol, or another humectant in order to assist in         retaining the final desired moisture content.     -   The tissue can be stretched, staked, and/or tumbled once or         repeatedly or left untreated     -   The tissue can be treated with a pigment or left untreated     -   The tissue is dried using a convection oven, is freeze dried,         air dried, or conductively dried

A specific example of the method employing a mycological biopolymer made in accordance with a method described in US 2015/0033620 and an organic solvent solution is as follows:

Example 1

-   1. An 18-inch by 11-inch by 2.5-inch panel of a mycological     biopolymer (“precursor tissue”) is grown and extracted from a     substrate composed of 15% crude protein, 33% non-fiber     carbohydrates, 28% lignin and 14% crude fat. The remaining 2%     includes mineral content, and the 8% is native moisture content. -   2. The wet live tissue is cut into 5-inch by 5-inch by 2.5-inch     sections. -   3. Each tissue section is placed in a container and submerged in an     organic solvent, such as a 1500 ml bath of 100% alcohol such as     isopropyl, ethanol, methanol, and the like. Each section is left in     this solution for 7 days. The sections are then removed from the     baths and the same process is repeated once for each panel section. -   4. The tissue sections are removed from the alcohol baths and     immediately pressed between a pair of rollers to 0.125-inches. -   5. The tissue sections are left on drying racks in a fume hood or     well ventilated area to air dry.

In the embodiment employing an organic solvent and salt solution, the following steps are performed:

-   1. A panel of wet live tissue or dried tissue, i.e., a precursor     tissue, with or without the substrate can be used. -   2. The tissue can be treated with lipids and/or     moisturizing/hydrating agent once or repeatedly, or left untreated     at any point throughout this process. -   3. The tissue can be sectioned or left intact to allow for a variety     of manufacturing sizes. -   4. The tissue can be treated (via submersion, vacuum infusion,     and/or injection) once or repeatedly with organic solvent solutions     for 5 seconds to 6 months prior to and/or after process step 5 or     left untreated. 5-50 ml of solution per 1 g of panel should be used     for each treatment. -   5. The tissue is treated (via submersion, vacuum infusion,     injection, etc.) once or repeatedly with 20 to 300 g/l salt and     organic solvent solutions for 5 seconds to 6 months. 5-50 ml of     solution per 1 g of panel should be used for each treatment. -   6. The tissue, after removal from the substrate if still tethered to     the substrate, is compressed using a manual press, hydraulic press,     or rollers. Pressing can be a hot or cold process. This is a means     of mechanically expelling any of the residual fluid and setting the     thickness, since the mycelium can plump during treatment. It is     important to set the thickness immediately following the treatment     to reduce rebound and shrinkage (e.g., fixation) -   7. The tissue can be dried using a convection oven, can be freeze     dried, air dried, or conductively dried -   8. The tissue can be treated with a plasticizer which could include     glycerin, sorbitol, or another humectant in order to assist in     retaining the final desired moisture content. -   9. The tissue can be stretched, staked, and/or tumbled once or     repeatedly or left untreated -   10. The tissue can be treated with a pigment or left untreated.     Should the tissue be dyed, steps 10 and 8 would be swapped. -   11. The tissue is dried using a convection oven, is freeze dried,     air dried, or conductively dried

A specific example of the method employing a panel of mycological biopolymer made in accordance with a method described in US 2015/0033620 and an organic solvent and salt solution 13 in a container 14 as shown in FIG. 1 is as follows:

Example 2

-   1. An 18-inch by 11-inch by 2.5-inch panel of precursor mycological     biopolymer is grown and extracted from the substrate composed of 15%     crude protein, 33% non-fiber carbohydrates, 28% lignin and 14% crude     fat. The remaining 2% includes mineral content, and the 8% is native     moisture content. -   2. The wet live tissue is cut into 5-inch by 5-inch by 2.5-inch     sections. -   3. An organic solvent and salt solution 13 of 150 g/L CaCl2 in 100%     alcohol (isopropyl, ethanol, methanol, and the like) is prepared and     placed in the container 14 (FIG. 1 ) and each section 15 is     submerged in 1500 ml bath of this solution. The container 14 is then     sealed and each section 15 is left in this solution for 7 days. The     sections 15 are then removed from the baths and the same process is     repeated twice for each panel section for a total of 3 consecutive     solution baths over 21 days. Alternatively, the solution could be.     agitated to accelerate the process time. These agitation methods     include stirring, wave motions, tumbling in a drum, and the like.     Mild heat could be applied, not to exceed 40° C. -   4. Sections 15 are removed from the CaCl2 and alcohol solution and     pressed to 0.5 inches using two pairs of spaced apart rollers 11 as     in FIG. 3 . The rollers 11 may be operated manually in the manner of     a wringer. -   5. A solution of 100% alcohol (isopropyl, ethanol, methanol, etc.)     (not shown) is prepared and each tissue section 15 is submerged in     1500 ml of this solution. Each tissue section 15 is left in this     solution for 3 days. -   6. The sections 15 are removed from the alcohol baths and     immediately pressed, for example, using the rollers 11 of FIG. 3     adjusted to reduce the thickness of the sections to 0.125 inches. -   7. The sections 15 are left on drying racks (not shown) in a fume     hood or well ventilated area to air dry.

FIG. 5 illustrates a flow diagram of the entire treatment process of Example 2 for organic solvent and salt solutions.

In the embodiment employing an organic solvent and a phenol and/or polyphenol substance solution, the following steps are performed:

-   1. A panel of wet live tissue or dried tissue, i.e., a precursor     tissue, can be used. -   2. The tissue can be treated with lipids and/or     moisturizing/hydrating agent once or repeatedly, or left untreated     at any point throughout this process. -   3. The tissue can be sectioned or left intact to allow for a variety     of manufacturing sizes. -   4. The tissue with/without substrate can be treated (via submersion,     vacuum infusion, injection, and the like) once or repeatedly with     organic solvent solutions for 5 seconds to 6 months prior to and/or     after process step 5 or left untreated. 5-50 ml of solution per 1 g     of panel should be used for each treatment. -   5. The tissue is treated (via submersion, vacuum infusion,     injection, etc.) once or repeatedly with organic solvent and phenol     and/or polyphenol solutions for 5 seconds to 6 months. 5-50 ml of     solution per 1 g of panel should be used for each treatment. -   6. The tissue (without substrate) is compressed using a manual     press, hydraulic press, or rollers. Pressing can be a hot     (temperature of 140° F.) or cold process. This is a means of     mechanically expelling any of the residual fluid and setting the     thickness, since the mycelium can plump during treatment. It is     important to set the thickness immediately following the treatment     to reduce rebound and shrinkage (e.g., fixation). -   7. The tissue can be dried using a convection oven, can be freeze     dried, air dried, or conductively dried. -   8. The tissue can be treated with a plasticizer which could include     glycerin, sorbitol, or another humectant in order to assist in     retaining the final desired moisture content. -   9. The tissue can be stretched, staked, and/or tumbled once or     repeatedly or is left untreated. -   10. The tissue can be treated with a pigment or left untreated. -   11. The tissue is dried using a convection oven, is freeze dried,     air dried, or conductively dried.

A specific example of the method employing a panel of mycological biopolymer made in accordance with a method described in US 2015/0033620 and an organic solvent and phenol and/or polyphenol solution 16 in a container 17 as shown in FIG. 2 wherein tannic acid, a polyphenolic compound, is used is as follows:

Example 3

-   1. An 18-inch by 11-inch by 2.5-inch panel of mycological biopolymer     is grown and extracted from the substrate composed of 15% crude     protein, 33% non-fiber carbohydrates, 28% lignin and 14% crude fat.     The remaining 2% includes mineral content, and the 8% is native     moisture content. -   2. The wet live tissue is cut to 5-inch by 5-inch by 2.5-inch     sections 18. -   3. The tissue is compressed to 0.125-inches via a hydraulic press. -   4. A solution of 5% acetic acid, such as vinegar, is prepared and     each tissue section 18 is submerged in 10,000 ml of this solution.     Each tissue section 18 is left in this solution for 24 hrs. to bring     the pH of said tissue section to a neutral to acidic pH of 5 to 7 to     support dying and crosslinking; -   5. The sections are then removed from the acid baths, rinsed in     10,000 ml of water for 1 minute, and manually pressed via wringing     of the tissue. -   6. A solution 16 of 10 g/l Tannic acid powder and water is prepared     and each tissue section 16 is submerged in 10,000 ml of this     solution 16. Each section 18 is left in this solution for 7 days.     (See FIG. 2 ) -   7. The sections 18 are then removed from the tannic acid baths,     rinsed in 10,000 ml of water for 1 minute, and manually pressed via     wringing of the tissue. -   8. A solution of 20 g/l Tannic acid powder and water is prepared,     and each tissue section 18 is submerged in 10,000 ml of this     solution. Each section 18 is left in this solution for 14 days. -   9. The sections 18 are then removed from the tannic acid baths,     rinsed in 10,000 ml of water for 1 minute, and manually pressed via     wringing of the tissue, e.g., as indicated in FIG. 3 . -   10. A solution of 20 (g/l) vegetable glycerin and water is prepared,     and each tissue section 18 is coated in 100 ml of this solution. -   11. The tissue sections 18 are mechanically agitated via stretching     and/or tumbling of material until the sections 18 are between 20-30%     moisture -   12. The tissue sections 18 are each coated in 50 ml of 20 g/l     vegetable glycerin and water solution and mechanically agitated     until the sections are between 20-30% moisture. This process is     repeated until the sections 18 reach a desirable flexibility     determined through bend radius, i.e., the material's ability to wrap     around a 1″ outer diameter rigid tube forming a 180° bend about the     tube without cracking.

FIG. 4 illustrates a coated tissue section 18 with dimensions of 5 inches by 5 inches by 0.125 inches being twisted longitudinally over an angle of 360°.

-   13. The tissue sections 18 are tumbled and air dried. The sections     18 can be draped or pressed with a match mold over a buck to provide     a geometry during the drying process.

In the embodiment employing a solution of an organic solvent combined with a phenol and/or polyphenol substance as well as with a solution of an organic solvent combined with a salt, such as calcium chloride, the following steps are performed:

-   1. A panel of wet live tissue or dried tissue, i.e., a precursor     tissue, can be used -   2. The tissue can be treated with lipids and/or     moisturizing/hydrating agent once or repeatedly, or left untreated     at any point throughout this process -   3. The tissue can be sectioned or left intact to allow for a variety     of manufacturing sizes. -   4. The tissue with/without substrate can be treated (via submersion,     vacuum infusion, injection, etc.) once or repeatedly with organic     solvent solutions for 5 seconds to 6 months prior to and/or after     process step 5 and 6 or left untreated. 5-50 ml of solution per 1 g     of panel should be used for each treatment. -   5. The tissue with/without substrate is treated (via submersion,     vacuum infusion, injection, etc.) once or repeatedly with organic     solvent and phenol and/or polyphenol solutions for 5 seconds to 6     months prior to and/or after process step 5-50 ml of solution per 1     g of panel should be used for each treatment. -   6. The tissue with/without substrate is treated (via submersion,     vacuum infusion, injection, etc.) once or repeatedly with 20 to 300     g/L salt and organic solvent solutions for 5 seconds to 6 months.     5-50 ml of solution per 1 g of panel should be used for each     treatment. -   7. The tissue (without substrate) is compressed using a manual     press, hydraulic press, or rollers. Pressing can be a hot or cold     process. -   8. The tissue can be dried using a convection oven, can be freeze     dried, air dried, or conductively dried -   9. The tissue can be treated with a plasticizer which could include     glycerin, sorbitol, or another humectant in order to assist in     retaining the final desired moisture content. -   10. The tissue can be stretched, staked, and/or tumbled once or     repeatedly or is left untreated -   11. The tissue can be treated with a pigment, or left untreated -   12. The tissue is dried using a convection oven, is freeze dried,     air dried, or conductively dried

A specific example of the method employing a panel of mycological biopolymer made in accordance with a method described in US 2015/0033620 and an organic solvent and calcium chloride solution and an organic solvent and phenol and/or polyphenol solution is as follows:

Example 4

-   1. An 18-inch by 11-inch by 2.5-inch panel of mycological biopolymer     is grown and extracted from the substrate composed of 15% crude     protein, 33% non-fiber carbohydrates, 28% lignin and 14% crude fat.     The remaining 2% includes mineral content, and the 8% is native     moisture content. -   2. The wet live tissue is cut to 18-inch by 5-inch by 2.5 inch     sections. -   3. The tissue sections are compressed to a thickness of 0.5 inches     via hydraulic press -   4. A solution of 10 g/l Tannic acid powder and water is prepared,     and each section is submerged in 5,500 ml of this solution. Each     section is left in this solution for 7 days (FIG. 2 ). -   5. A solution of 150 g/l CaCl2 in 100% alcohol (isopropyl, ethanol,     methanol, and the like) is prepared and each tissue section is     submerged in 5,500 ml of this solution. Each section is left in this     solution for 7 days. The sections are then removed from the baths     and the same process is repeated once for each panel section for a     total of 2 consecutive solution baths over 14 days. (FIG. 1 ). -   6. Tissue sections are removed from the CaCl2 and alcohol solution     and pressed to 0.5-inches using a roller. (FIG. 3 ). -   7. A solution of 100% alcohol (isopropyl, ethanol, methanol, etc.)     is prepared and each pressed tissue section is submerged in 5,500 ml     of this solution. Each section is left in this solution for 1 day. -   8. The tissue sections are removed from the alcohol baths and     immediately pressed using a pair of rollers to 0.125-inches (FIG. 3     ). -   9. The tissue sections are left on drying racks in a fume hood or     well ventilated area to air dry. -   10. A solution of 20 (g/L) vegetable glycerin and water is prepared,     and each tissue section is coated in 100 ml of this solution -   11. The tissue sections are mechanically agitated via stretching     and/or tumbling of material until the sections have reached a     desired softness and flexibility. -   12. The tissue sections are tumbled and air dried. Tumbling will     loosen the mycelium fibers and assist with achieving the desired     hand.

A specific example of the method employing a panel of mycological biopolymer made in accordance with a method described in US 2015/0033620 and a solution of tannins is as follows:

Example 5

-   Steps 1-9 as cited in Example 4 -   Process in which the precursor tissue is then placed in a solution     of tannins, in which the tannins are applied at 5% of the dry tissue     mass with a 1:100 ratio with municipal tap water. -   The processed tissue is then dried using forced convection at 180 F. -   The processed tissue is then dyed with the dye being applied at 5%     of the dry tissue mass with a 1:100 ratio with municipal tap water. -   The processed tissue is then rinsed with an acetic acid solution at     a pH of 3 to fix the dye. -   The processed tissue is then rinsed with municipal tap water to     remove any unfixed dye. -   The processed tissue is then dried using forced convection at 180 F. -   The processed tissue is embossed to provide a surface pattern. -   The processed tissue is spray coated with a film of wax to prevent     water penetration.

The solution of tannins (i.e., an organic solvent solution) may be composed of any of various soluble astringent complex phenolic substances of plant origin used especially in tanning leather and dyeing textiles.

The above described post-processing treatments of a known mycological biopolymer material as a precursor tissue serve to enhance the inherent material properties of the material.

In this instance, the treatment fixes the precursor tissue, making the tissue more durable to repeat stress, resistant to microbial decay, and resistance shear stress (tearing). This retains the properties of the extracted mycelium (wet) over tissue that has been actively dried which has been shown to embrittle the material, specifically retaining elasticity and toughness.

The treatment of the tissue with solvent will enable penetration, rinse extracellular materials away, denature proteins, and deacetylate. The latter two post treatments open sites for crosslinking and fixation.

The treatment of the tissue with phenol provides crosslinking agents, and specifically provides covalent bonds between the primary amine of chitin and the amines and hydroxyl of amino acid residues.

The salt is a humectant and antimicrobial agent. Coupled with methanol, calcium chloride deacetylates chitin which mediates bond formation. In water, the salt can form ionic bonds with the same functional groups.

The pre-processed precursor mycological biopolymer material may be made as described in US 2015/0033620 or may be obtained from any suitable source so long as the material is made of undifferentiated fungal mycelium, specifically a chitin-polymer where extracellular matrix has been rinsed away.

Further, the pre-processed precursor mycological biopolymer material provided for post-processing treatment may have other materials incorporated therein depending upon the ultimate use of the post-processed material, for example, the pre-processed material may have heat insulating particles or elements incorporated therein where the ultimate use of the post-processed material is for heat insulation purposes. There could be embedded materials, such as particles that provide a thermal conductivity benefit, or a structural member, such as a scrim.

The invention thus provides a processed mycological biopolymer material of increased elasticity, strength and density as compared to previously known mycological biopolymers.

The invention also provides a mycological biopolymer that is a tough pliable material that can be used to replace, textiles, leather, and leather-like materials, such as, polyurethane, silicone, and poly vinyl acetate coated scrims and that provides a high density foam like material for use in upholstery, apparel, military gear, athletic gear, and footwear. 

1. A mycological biopolymer material consisting essentially of a fungal mycelium free of any stipe, cap, or spores, wherein the mycological biopolymer material has a density in a range of 15 pcf to 50 pcf.
 2. The mycological biopolymer material of claim 1, wherein the mycological biopolymer material has an elasticity in a range of 2000 psi to 8000 psi.
 3. The mycological biopolymer material of claim 1, wherein the mycological biopolymer has a thickness less than 1.25 inches. 4-21. (canceled)
 22. The mycological biopolymer material of claim 3, wherein the thickness is between 0.125 inches and 1.25 inches.
 23. The mycological biopolymer material of claim 1, wherein the mycological biopolymer material has a porosity in a range of 0.9 micrometers to 25.0 micrometers.
 24. The mycological biopolymer material of claim 1, wherein the mycological biopolymer material has a native moisture content of greater than 15%.
 25. The mycological biopolymer material of claim 1, wherein the mycological biopolymer material has a native moisture content between 15-30%.
 26. The mycological biopolymer material of claim 1, wherein the mycological biopolymer material is resistant to microbial decay.
 27. The mycological biopolymer material of claim 1, wherein the mycological biopolymer material is at least one of bleached and odorless.
 28. A processed mycological biopolymer material comprising the mycological biopolymer material of claim 1, further comprising a dye.
 29. A processed mycological biopolymer material comprising the mycological biopolymer material of claim 1, further comprising a wax coating.
 30. A processed mycological biopolymer material comprising the mycological biopolymer material of claim 1, further comprising an embossed pattern on a surface of the mycological biopolymer material.
 31. A processed mycological biopolymer material comprising the mycological biopolymer material of claim 1, further comprising a plurality of thermally conductive particles.
 32. A processed mycological biopolymer material comprising the mycological biopolymer material of claim 1, further comprising one or more structural members.
 33. The processed mycological biopolymer material of claim 32, wherein the one or more structural members are scrims.
 34. A processed mycological biopolymer material comprising the mycological biopolymer material of claim 1, further comprising a plasticizer coating.
 35. The processed mycological biopolymer material of claim 34, wherein the plasticizer coating comprises a humectant.
 36. The processed mycological biopolymer material of claim 35, wherein the humectant comprises at least one of glycerine and sorbitol
 37. The processed mycological biopolymer material of claim 36, wherein the glycerine comprises a vegetable glycerine.
 38. A processed mycological biopolymer material comprising the mycological biopolymer material of claim 1, further comprising at least one of a tannin residue and a salt residue.
 39. A textile, leather-like or foam-like material comprising the mycological biopolymer material of claim
 1. 40. An apparel comprising the textile of claim
 39. 41. A scrim, athletic gear, upholstery, apparel, military gear, or footwear comprising the mycological biopolymer material of claim
 1. 